Pipette Blog

Transfer pipettes serve the scientific community’s need to move liquids between containers or onto microscope slides. Size and quality vary greatly, from small, cheap, plastic transfer pipettes at the high-school level to highly precise glass pipettes with separate bulbs seen in professional laboratories. Regardless of its construct, using a transfer Pipette Tips is a simple task once the user gets accustomed to the procedure.
1 Hold the pipette with your fingers near the top with your dominant hand. Ensure your index finger can move without dropping the pipette.
2 Grab the pipette bulb in your free hand and squeeze it. Place the nozzle of the bulb, called the taper, onto the top of the pipette.
3 Gently put the pipette tip into deionized water. Move the pipette into a vertical position and release your squeeze on the bulb. The water should start to rise up the pipette. If it doesn’t, gently wiggle the bulb until it does.
4 Fill the pipette to about 1/3. Remove the pipette bulb and quickly replace it with your index finger.
5 Pull the pipette out of the water and hold it horizontally. Remove your finger from the hole. Hold the pipette with both hands and rotate it. Coat the entire inner surface of the pipette with water.
6 Place your index finger back on the top of the Transfer Pipettes and hold it vertically. Tilt a waste beaker so the pipette is touching its inside. Release your index finger to allow the water to drain out. Hold it vertically over the beaker for 10 seconds to ensure all the water has drained.
7 Place the bulb onto the top of the pipette and squeeze it to blow out any additional water. Repeat Steps 1- 7 with the deionized water two times and three times with the liquid you want to transfer. The pipette is now clean.
8 Squeeze the pipette bulb with your nondominant hand and put it on the top of the pipette. Put the pipette into the solution. Place the tip in the solution you want to transfer and release the bulb.
9 Place the end of the pipette gently on the bottom of the beaker if the pipette hasn’t filled by the time the bulb is fully released. Place your index finger on the opening and squeeze the pipette bulb. Replace your index finger with the pipette bulb and release until the pipette is full. Fill the pipette to a few centimeters above the top graduation.
10 Replace the pipette bulb and then pull the pipette out of the solution. Dry the outside of the pipette with a chemical wipe. Tip the beaker and place the pipette on the side. Release your index finger gradually until the liquid reaches the top graduation.
11 Touch the tip of the pipette to the top of the beaker to remove any remaining drops. Transfer the liquid to the receiving vessel.
12 Place the pipette tip to the side of the receiving vessel and drain by lifting your finger. Hold the pipette there for 10 additional seconds. Touch the tip to a clean location on the receiving vessel to remove excess liquid.
13 Rinse the pipette three times with tap water by soaking it up from a beaker. Rinse it an additional three times with deionized water before letting it dry.
The article comes from Plastic Pipettes.

In molecular biology where a lot of chemical and cell tests are required, Serological Pipettes become imperative in the research. Pipettes are the most useful tool to extract the right amount of samples and introduce the samples into the corresponding laboratory machine. They measure the sample with accuracy and precision that is beyond doubt.
1 Hold the pipette vertically at eye level after you have drawn up your liquid. If there’s a table nearby, stabilize the butt of the pipette against the table and bend down so that the pipette is still at eye level.
2 Find the level of the liquid inside the pipette. The surface of the liquid will look as though it curves. This is called a “meniscus curve,” and it occurs because the water molecules experience mild attraction and adhesion to the sides of the pipette.
3 Find the black measurement line that meets the bottom of the meniscus curve. In many serological Transfer Pipettes, each line represents one tenth of a millimeter; others are more accurate, with lines representing one hundredth of a millimeter.
4 Count up the number of lines from the nearest whole number below the bottom of your meniscus curve. Record your reading with the appropriate units. For example, if you see a number 3 and four black lines, record 3.4 and the units.
The article comes from Pipette.

Serological Pipettes have gradations that continue down into the tips. Additionally, some serological pipettes are also blow-out pipettes. These pipettes have an open top, much like a straw, where the user holds his thumb over the top to create the vacuum and seal the liquid in the pipette. Blow-out pipettes allow you to blow into this open end to get the last bits of liquid left in the pipette into your receiving container, for greater accuracy. Blow-out pipettes are clearly marked with a frosted band or two thin rings around the neck. Do not mistake a color coding from a manufacturer for the markings of a blow-out pipette. While you can use blow out pipettes in their intended manner, it is dangerous to do this with a pipette that is not clearly marked as being a blow-out.
1 Hold the pipette with Pipette Tips vertically at eye level after you have drawn up your liquid. If there’s a table nearby, stabilize the butt of the pipette against the table and bend down so that the pipette is still at eye level.
2 Find the level of the liquid inside the pipette. The surface of the liquid will look as though it curves. This is called a “meniscus curve,” and it occurs because the water molecules experience mild attraction and adhesion to the sides of the pipette.
3 Find the black measurement line that meets the bottom of the meniscus curve. In many serological pipettes, each line represents one tenth of a millimeter; others are more accurate, with lines representing one hundredth of a millimeter.
4 Count up the number of lines from the nearest whole number below the bottom of your meniscus curve. Record your reading with the appropriate units. For example, if you see a number 3 and four black lines, record 3.4 and the units.
The article comes from Pipette.