How the Test Works
Soybeans that have been genetically modified with the CP4 EPSPS gene will express Roundup herbicide resistance in the plant and seed. The QuickStix kit detects the CP4 EPSPS protein produced in the seed. To conduct the test, a seed sample is placed in a small ziplock plastic bag and crushed with a pliers. Then the proteins that may be present in the seed are solubilized in water where they can be detected by a lateral flow immunochromatographic test. This assay uses antibodies on the strips to detect the presence of the Roundup Ready protein in the seed. A control line will appear on each strip. If Roundup Ready protein is present in the seed, the reaction shows a color change on the strip producing a second test line.

Each EnviroLogix QuickStix test strip has an absorbent pad at each end. The protective tape with the arrow indicates the end of the strip to insert into the extraction tube. The water with the dissolved proteins (if present) will travel up the membrane strip and be absorbed in the larger pad at the top of the strip. The portion of the strip between the protective tape and the absorbent pad at the top of the strip is used to view the reactions as described in the "Interpreting the Results" section of these instructions.

Pre-Lab
The instructor should set out two containers labeled A and B. One container will have soybeans that express the Roundup Ready protein. A second container of soybeans will not express the Roundup Ready protein. Several groups should test identical containers of seeds to confirm results.

Have the students observe the seeds and then record a hypothesis about which seeds they believe to be genetically modified.

NOTE: Be very careful to prevent sample-to-sample cross-contamination of seeds, fluids, or disposables. Repeat the protocol for each sample to be tested, using a new tube and plastic bag for each.