A Plastic Funnel is used to pour materials into containers that have narrow openings. Funnels are shaped like a modified pipe with one narrow end and one wide end and may be made of plastic, metal, glass or other substances depending on the intended use. You can use funnels to pour liquids or pourable solid materials such as sand into receiving containers. Regardless of the material being poured, the proper way to use a funnel is the same.
1 Place the narrow end of the funnel into the top of the receiving container. Depending on the size and stability of the funnel and the container, you may need to hold the funnel in place with one hand.
2 Pour the material into the wide end of the funnel. Watch as you pour to ensure that the material passes through the narrow end of the funnel faster than you add the materials to the wide end. This prevents the substance from overflowing from the wide end of the funnel.
3 Angle the pouring container away from you as you pour the material into the funnel. This helps ensure that the material will not splash you or your clothing.
4 Monitor the amount of material you have poured through the funnel into the receiving container. When the receiving container is full, the material will no longer pass through the narrow end of the funnel and will overflow the wide end of the funnel if you continue pouring it.
5 Pull the funnel out of the receiving container when you finish pouring material into it. Take care when removing the funnel to avoid dripping material from the narrow end of the funnel onto the exterior of the receiving container. Clean the funnel according to the manufacturer's directions.
Streak a Plastic Petri Dishes
1 Find an open and clean area of bench, making sure to disinfect it entirely before you begin. Ignite the Bunsen burner and flame the wire loop until it's heated red, then allow it to cool for a few minutes upright in a rack. Repeat this process for three or so more wire loops.
2 Dip the sterilized loop in the culture broth. Delicately spread the liquid across the agar surface in a straight line along the edge of the Petri dish. Spread three more lines with the same loop to make four parallel lines in the agar and quickly cover the Petri dish with a lid.
3 Flame the loop with the Bunsen burner and allow a couple minutes for it to cool. Rotate the Petri dish slightly to start a new set of lines. This new set should intersect with the end half of the first set of lines along the edge of the Petri dish. Grab another sterilized loop and make the new set of parallel lines, then cover the dish quickly with the lid.
4 Repeat the process for two more sets of parallel lines around the remaining edge space of the Petri dish, promptly covering the dish with its lid after each set. For the final line in the fourth set, make a zigzag pattern in the dish.
5 Close and seal the Petri dish. Write a label including the culture type, your name, and the date and store in an incubator upside down.
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