Pipette tip is a very important part of pipette.
At the tip (pipette tips) put pipette, many people turned their guns on the box hard knock in a few, then wrong, as this would result in the internal pipette accessories (such as spring) due to instantaneous impact force generated percussion have become loose, and even lead to scale adjustment knob stuck. Correct method is to pipette (control) into the tip in the vertical, rotating slightly around a little hard to make it closely. If multi-channel (such as 8 or 12) pipette, the pipette can be the first at the first tip, and then obliquely inserted, after the forward direction of shaking can be locked in place. Tip card is a little more than a sign of tight O-ring, and you can see part of the formation of clear ring connection.Plastic Funnel
Pipette method
1) Pre-washing tips:
We installed a new tip or increase the capacity value of the future, should the need to transfer the liquid draw, emissions two to three times, doing so is to enable the tip to form a homogeneous liquid film wall to ensure the accuracy of pipetting work and accuracy, the entire pipette with high reproducibility. Second, learning from high-volatile organic solvents or liquid, volatile gases will form a negative pressure room in the white sleeve, resulting in leakage of the case, then we need four to six pre-washed, so the white interior of the sleeve gas saturation, negative pressure will automatically disappear.
2) aspiration:
First pipette according to the first stop button discharge point, and then immersed in liquid suction head vertically, the depth of immersion is: P2, P10 less than or equal to 1 mm, P20, P100, P200 less than or equal to 2 mm, P1000 is less than or equal to 3 mm, P5ML, P10ML less than or equal to 4 mm (immersed in too deep, then the hydraulic fluid will absorb a certain precision, of course, the specific liquid immersion depth should be based on the container in full bloom size flexibility), a smooth release the button, remember not too fast.
Before pipetting, to ensure that pipette tip and the liquid at the same temperature. Absorb the liquid, the pipette to keep upright state,Plastic Dropper Bottles
Then you can take two pipette method.
First, the forward pipetting method. Press the button with the thumb to the first stopping point, then slowly release the button back to the origin. Then press the button to drain fluid first stopping point, pause for a moment to press the button to the second stop point blow residual liquid. Finally, release the button.
Second, reverse pipetting method. This method is generally used to transfer high viscosity liquid, bio-active liquid, foaming liquid or very small amount of liquid, its principle is to first set the range of inhalation than liquid, do not blow the transfer of liquid when the residual liquid. First press the button to the second stopping point, slowly release the button to the origin. Then press the button to set the first stopping point range of liquid discharge, continue to hold the buttons in the first stopping point (Do Do not press down), remove residual liquid tip, abandoned.
3) put the solution:
Subretinal fluid, the tip close to the container wall, the first button press to discharge the first stopping point, after a brief pause, and then to the second stop point, which could ensure that no residual fluid within the tip. If this operation there is still residual liquid, then you should consider replacing the suction head.
4) Remove the suction head:
Remove the tip and the new tip must not mix, so as to avoid cross-contamination.Specimen Containers
