Adjustment range
Range in the regulation, if a large volume of notes from a small volume, adjusted in accordance with normal methods, you can rotate the knob; but if you want to transfer to a large volume of small size, the scale may be the first knob clockwise to more than range of the scale, adjusted to set the volume back, so you can take to ensure the maximum amount of accuracy. In the process, do not spin out of range of the button, otherwise it will get stuck and damage the internal mechanism of the pipette gun.
Assembly tips (suction nozzle)
Pipette tips will be put in the gun, the correct method is to pipette gun (control) into the tips of the vertical, a slight rotation around a little harder to make it closely. If it is multi-channel (eg, 8 or 12) pipette gun, the gun can be the first pipetting at the first tip, and then obliquely inserted, after the forward direction of shaking to clamping. Tips card is slightly more than a sign of tight O-ring, and you can see part of the formation of clear ring connection.
Pipette method
Before pipetting to ensure that pipettes, tips and liquid at the same temperature. Absorb liquid, the pipette to keep upright state, the tips into the liquid under the 2-3 mm. Before the suction can be absorbing and releasing several times the suction of liquid to moisten the mouth (in particular, to draw water viscosity or density of different liquids.) Then you can take two pipette method.
First, forward pipetting method.
Press the button with the thumb to the first stopping point, then slowly release the button back to the origin. Then press the button to drain fluid first stopping point, pause a moment to press the button to the second stopping point blow residual liquid. Finally, release the button.
Second, reverse pipetting method. This method is generally used to transfer high viscosity liquid, biological activity of liquid, bubbling liquid, or a very small amount of liquid, the principle is to first set the range of more than inhalation of the liquid, do not blow out when the transfer of liquid remaining liquid. Click the button to the second stop point, slowly release the button to the origin. Then press the button to set the first stopping point range of liquid discharge, continue to hold the button in the first stopping point (Do Do not press down) and remove a residual liquid tip, discarded.
The correct placement pipettes
After use, it can be hung on a vertical pipette gun rack, but be careful not to fall. When the pipette tips in a liquid, not the pipette horizontally or upside down, so as to avoid corrosion of the liquid piston springs back.
Maintenance Precautions
If you do not use the gun range should pipetting scale adjusted to the maximum, the spring in a relaxed state to protect the springs. Regular cleaning pipette gun is best, you can use soapy water or 60% isopropanol, and then distilled water, air dry.
Pasteurized prior to ensure that the pipette can adapt to high temperatures.
Calibration is 20-25 degrees in the environment, repeated several times by distilled water approach to weighing.
Used to check whether there is leakage phenomenon. Method after drawing the liquid suspended vertically in a few seconds to see whether the drop surface. If the leakage, because there are basically about several aspects:
1, the tips match;
2, spring piston is normal;
3, if it is volatile liquids (organic solvents are so many), then the problem may be the saturation vapor pressure. Absorption and desorption can be several times the liquid, and then pipetting.
Assistant pipette device rechargeable lightweight, wireless and portable. With a charger, charging time can be 6-8 hours of continuous use, built-in 0.45 μ m membrane hydrophobic membrane filter to prevent liquid suction to protect the internal components. Assistant suction pipette can replace the traditional ball ear, for all 1-100 ml glass or plastic pipette. Wind speed of the suction pipette from the pump speed regulator control, the operator can use the size of pipette volume selection stalls, to speed up or slow down the absorption / wind speed. The main original device Assistant pipettes from Germany and the United States procurement, use of recyclable plastic materials. Pipette with a low environmental pollution, rechargeable 1.2-volt lithium battery.
1 with a variety of specifications supporting the use of pipette
2, rechargeable portable design
3 kinds of voltage specifications available: 110 and 220 volts
4, rechargeable portable design, and low environmental pollution, with a 1.2-volt rechargeable Ni-MH battery and charger, charging time 6-8 hours of continuous use
5, built-in 0.45μm hydrophobic filter to prevent inhalation of the liquid in order to protect internal components
6, the suction from the pump speed wind speed regulator control, the operator can use the capacity size of the selected pipette gear to speed up or slow down the suction / blowing velocity
7, with a variety of specifications supporting the use of Pipettes